Cell Culture Media
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BIOLIFE SOLUTIONS INC HypoThermosol® 10mL
This item has a minimum qty of 6 per supplier requirements.
HypoThermosol is an optimized hypothermic (2-8C) preservation media that enables improved and extended preservation of cells, tissues and organs. HypoThermosol is uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process. HypoThermosol includes key ions at concentrations that balance the intracellular state at hypothermic temperatures. Additional components include pH buffers, energy substrates, free radical scavengers, and osmotic/oncotic stabilizers.
NEOGEN Agar No. 1 Bacteriological, 500 g
Agar No. 1 Bacteriological is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.A high clarity agar with good gelling properties and a low concentration of metal ions. This agar is suitable for all bacteriological purposes including sensitivity testing and pour plate techniques. A firm gel is obtained at working concentrations of 1.0 to 1.5%. No significant precipitation is observed on reheating or prolonged holding at 65°C.For more information visit https://www.neogen.com/solutions/Microbiology/agar-number-1-bacteriological
NEOGEN Hektoen Enteric (HE) Agar, 500 g
Hektoen Enteric Agar is used for the isolation and differentiation of enteric pathogens. Hektoen Enteric Agar is not intended for use in the diagnosis of disease or other conditions in humans. Hektoen Enteric Agar was developed in 1967 by King and Metzger. Compared to other enteric differentiating media commonly used, Hektoen Enteric Agar increased the isolation rate of Salmonella spp. and Shigella spp. This was accomplished by increasing the carbohydrate and peptone content of the medium in order to counteract the inhibitory effects of bile salts and indicators. King and Metzger formulated a medium that slightly inhibited growth of Salmonella and Shigella, while inhibiting Gram-positive microorganisms. Enzymatic Digest of Animal Tissue provides nitrogen, carbon, and amino acids required for organism growth.For more information visit https://www.neogen.com/solutions/Microbiology/hektoen-enteric-agar
NEOGEN Oxford Listeria Agar, 500 g
Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water.For more information visit https://www.neogen.com/solutions/Microbiology/oxford-listeria-agar
NEOGEN Rose Bengal Chloramphenicol Agar, 500 g
Rose Bengal Chloramphenicol Agar is used for the selective isolation and enumeration of yeasts and molds from foods. Rose Bengal Chloramphenicol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Rose Bengal Chloramphenicol Agar Base is a selective medium for the enumeration of fungi. In 1944, Smith and Dawson used Rose Bengal for the selective isolation of fungi from soil samples. This formula was prepared with a neutral pH, deviating from the usual acidified medium. Several investigations have found a neutral pH with the addition of a selective agent have been successful in supporting fungal growth and restricting bacterial growth. Rose Bengal Agar pre-supplemented with Chloramphenicol is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis.For more information visit https://www.neogen.com/solutions/Microbiology/rose-bengal-chloramphenicol-agar
NEOGEN Potato Dextrose Agar, 5 kg
Potato Dextrose Agar is used for the preparation and maintenance of fungal test strains used in the growth promotion test, suitability of the counting methods and negative controls as described in the Harmonized USP/EP/JP. Potato Dextrose Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the cultivation of fungi and specifically for the preparation of the Aspergillus brasiliensis test strain. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to PDA. The extract from potato and dextrose provide a nutritionally rich base that encourages mold sporulation and pigment production. For more information visit https://www.neogen.com/solutions/Microbiology/potato-dextrose-agar
NEOGEN Fluid Thioglycolate Medium, 500g
Fluid Thioglycolate Medium is used for sterility testing. This medium conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans.Fluid Thioglycolate Medium is recommended by the Harmonized Pharmacopoeia for sterility testing and conforms to USP/EP/JP performance specification. In this formulation casein and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The glucose provides a carbon source and sodium chloride maintains osmotic balance. L-Cystine and sodium Thioglycolate act as reducing agents to create an anaerobic environment and maintain a low Eh. This is aided by the low level of agar which reduces the oxygen permeability through the medium. Resazurin is an oxidation indicator which turns from colorless to red/pink when oxidised.For more information visit https://www.neogen.com/solutions/fluid-thioglycollate-medium-ncm0108
NEOGEN BCYE Agar (Legionella Isolation Medium), 500 g
BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples. BCYE Agar is not intended for use in the diagnosis of disease or other conditions in humans.In 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires’ disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer.For more information visit https://www.neogen.com/solutions/Microbiology/bcye-agar-legionella-isolation-medium
NEOGEN Baird Parker Agar Base, 500 g
Baird Parker Agar is used for the cultivation of Staphylococcus aureus from foodstuffs according to ISO 6888, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally introduced in 1962, this medium was developed by Baird-Parker to overcome the problems of recovering damaged Staphylococcus aureus from foodstuffs. This version of the medium is formulated according to ISO 6888-1:1999+A1:2003 and is in compliance with ISO 6888-2:2003+A1:2003 and ISO 6888-3:2003.Baird-Parker medium is highly selective by nature, due to the presence of potassium tellurite and lithium chloride. Tellurite inhibits most coliforms and is also reduced to telluride by S. aureus, giving the typical black colonies. Glycine and sodium pyruvate are both used as growth factors by staphylococci while the pyruvate also neutralizes any toxic peroxides that may be formed.For more information visit https://www.neogen.com/solutions/Microbiology/baird-parker-agar-base
NEOGEN DRBC Agar (ISO), 500 g
Dichloran Rose Bengal Chloramphenicol Agar is for the enumeration of yeasts and is not intended for use in the diagnosis of disease or other conditions in humans.Developed with reference to ISO 21527-1:2008 for the enumeration of yeasts and molds in food and animal products, this medium is tested to the performance requirements of the standard. Used for the enumeration of viable yeasts and molds in products with a water activity of greater than 0.95 such as eggs, meat, some dairy products, fresh pastes, fruit and vegetables, DRBC Agar is designed to suppress the colonial growth of ‘spreader’ molds and in doing so allow easier performance of the colony count technique on yeasts and molds.For more information visit https://www.neogen.com/solutions/Microbiology/drbc-agar-iso
STEMCELL Technologies CryoStor CSB, undefined: 1000 mL
Reduce the level of cryopreservation-induced delayed-onset cell death and maximize post-thaw cell recovery, viability, and function following cryopreservation, with CryoStor CSB, an intracellular-like base formulation from the CryoStor preservation media series. Serum-free, animal component-free, and cGMP-manufactured, this chemically defined medium is sterile and tested for endotoxins and cell-based release. CryoStor CSB is formulated with USP-grade components and does not contain DMSO. Visit STEMCELL.com for more information.
NEOGEN LB Broth (Miller), 500 g
LB Broth (Miller) is used in molecular genetic studies in a laboratory setting. LB Broth, Miller is not intended for use in the diagnosis of disease or other conditions in humans. LB Broth (Miller) is based on the formula described by Miller. This medium is used for the growth and maintenance of Escherichia coli strains used in molecular microbiology procedures. LB Broth, Miller is a nutritionally rich medium designed for growth of pure cultures of recombinant strains. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to high copy numbers without selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol can be added to inhibit host synthesis and prevent replication of the bacterial chromosome. This broth contains a high level of sodium chloride to aid the maintenance of plasmids.For more information visit https://www.neogen.com/solutions/Microbiology/lb-broth-miller
NEOGEN MacConkey Broth Purple, 500 g
MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth-purple
NEOGEN Lauryl Sulfate Broth, 500 g
Lauryl Sulfate Broth is used for the detection of coliform bacteria in water and wastewater in a laboratory setting. Lauryl Sulfate Broth is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products, and other materials.Lauryl Sulfate Broth, also referred to as Lauryl Tryptose Broth, is prepared according to the formula of Mallmann and Darby. During their investigation, Sodium Lauryl Sulfate produced the best results for inhibition of organisms other than coliforms. For more information visit https://www.neogen.com/solutions/Microbiology/lauryl-sulfate-broth
NEOGEN A-1 Medium, 500 g
A-1 Medium is used for the detection of coliform organisms in water and food in a laboratory setting. A-1 Medium is not intended for use in the diagnosis of disease or other conditions in humans.Since the early 1900’s enumeration of coliform organisms, specifically E. coli, have been used to determine water purity. Elevated temperature and most-probable-number (MPN) methods are routinely used for analysis of water and food samples for presence of fecal coliforms. A-1 Medium was formulated to hasten E. coli recovery and reduce the incidence of false positive cultures.In 1972 Andrews and Presnell developed A-1 Medium. This medium recovers E. coli from estuarine water in 24 hours instead of 72 hours, and in greater numbers without the pre-enrichment step. A-1 Medium can be used in a single step procedure for the detection of fecal coliforms in source water, seawater, treated wastewater, and foods.For more information visit https://www.neogen.com/solutions/Microbiology/a-1-medium